Review



church lab grna vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Addgene inc church lab grna vector
    Church Lab Grna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/us12595478-4602-8-12
    Average 96 stars, based on 451 article reviews
    church lab grna vector - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Beyond Trypanosoma cruzi : LINE-1 Activation as a Driver of Chronic Inflammation in Chagas Disease
    Article Snippet: Plasmid extraction and purification were carried out using the InvitrogenTM PureLinkTM HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific ® , Waltham, MA, USA, catalog K210004), following the Midiprep protocol. .. The gRNA_Cloning Vector (Addgene #41824) was linearized using the restriction enzyme AflII, and oligonucleotides were inserted into the vector at a 10:1 molar ratio (oligo:vector) using the NEBuilderTM HiFi DNA Assembly Cloning Kit (New England BioLabs, Ipswich, MA, USA), in accordance with the manufacturer’s guidelines. ..

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination.
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. 76) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl−1) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Article Title: RNA-guided systems for in vivo gene editing
    Article Snippet: .. Confirmed protospacers were cloned into gRNA cloning vector (world wide website addgene.org/41824/) using overlap extension PCR. .. Different pairs of guide RNA plasmids flanking exon 23 or exons 52 and 53 were co-transfected with Sp hCas9_p2A GFP encoding plasmid into mouse C2C12 myoblast cells using lipofectamine 2000 Reagent (Invitrogen) according to the manufacturer's instructions.

    Article Title: Dynamic allele usage of X-linked genes ameliorates neurodevelopmental disease phenotypes in brain organoids
    Article Snippet: Cells were resuspended in 100 μl electroporation buffer P3 (P3 Primary cell solution box, Lonza) plus 2.5 μg of the CAG-Cas9-Venus plasmid (pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA was a gift from Ralf Kuehn (Addgene plasmid # 86986)) and 2.5 μg of gRNA-containing plasmid. .. The CAG-Cas9-Venus plasmid did not contain any gRNAs, but the gRNAs were provided by using the gRNA cloning vector (gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824)). .. Fibroblasts were reprogrammed into iPSCs with retroviruses and by using the feeder-dependent approach of the CytoTune(TM)-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher) according to the manufacturer’s protocol.

    Article Title: Beyond Trypanosoma cruzi: LINE-1 Activation as a Driver of Chronic Inflammation in Chagas Disease
    Article Snippet: Plasmid extraction and purification were carried out using the InvitrogenTM PureLinkTM HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific®, Waltham, MA, USA, catalog K210004), following the Midiprep protocol. .. The gRNA_Cloning Vector (Addgene #41824) was linearized using the restriction enzyme AflII, and oligonucleotides were inserted into the vector at a 10:1 molar ratio (oligo:vector) using the NEBuilderTM HiFi DNA Assembly Cloning Kit (New England BioLabs, Ipswich, MA, USA), in accordance with the manufacturer’s guidelines. ..

    Article Title: A novel role for the CNTN6 locus in lumenization and radial glial cell fate determination during early human cortical development revealed in cerebral organoids
    Article Snippet: .. Guide RNAs were cloned into a gRNA cloning vector (Addgene, #41824). ..

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. ) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl −1 ) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Cloning:

    Article Title: Beyond Trypanosoma cruzi : LINE-1 Activation as a Driver of Chronic Inflammation in Chagas Disease
    Article Snippet: Plasmid extraction and purification were carried out using the InvitrogenTM PureLinkTM HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific ® , Waltham, MA, USA, catalog K210004), following the Midiprep protocol. .. The gRNA_Cloning Vector (Addgene #41824) was linearized using the restriction enzyme AflII, and oligonucleotides were inserted into the vector at a 10:1 molar ratio (oligo:vector) using the NEBuilderTM HiFi DNA Assembly Cloning Kit (New England BioLabs, Ipswich, MA, USA), in accordance with the manufacturer’s guidelines. ..

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination.
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. 76) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl−1) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Article Title: RNA-guided systems for in vivo gene editing
    Article Snippet: .. Confirmed protospacers were cloned into gRNA cloning vector (world wide website addgene.org/41824/) using overlap extension PCR. .. Different pairs of guide RNA plasmids flanking exon 23 or exons 52 and 53 were co-transfected with Sp hCas9_p2A GFP encoding plasmid into mouse C2C12 myoblast cells using lipofectamine 2000 Reagent (Invitrogen) according to the manufacturer's instructions.

    Article Title: Dynamic allele usage of X-linked genes ameliorates neurodevelopmental disease phenotypes in brain organoids
    Article Snippet: Cells were resuspended in 100 μl electroporation buffer P3 (P3 Primary cell solution box, Lonza) plus 2.5 μg of the CAG-Cas9-Venus plasmid (pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA was a gift from Ralf Kuehn (Addgene plasmid # 86986)) and 2.5 μg of gRNA-containing plasmid. .. The CAG-Cas9-Venus plasmid did not contain any gRNAs, but the gRNAs were provided by using the gRNA cloning vector (gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824)). .. Fibroblasts were reprogrammed into iPSCs with retroviruses and by using the feeder-dependent approach of the CytoTune(TM)-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher) according to the manufacturer’s protocol.

    Article Title: Beyond Trypanosoma cruzi: LINE-1 Activation as a Driver of Chronic Inflammation in Chagas Disease
    Article Snippet: Plasmid extraction and purification were carried out using the InvitrogenTM PureLinkTM HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific®, Waltham, MA, USA, catalog K210004), following the Midiprep protocol. .. The gRNA_Cloning Vector (Addgene #41824) was linearized using the restriction enzyme AflII, and oligonucleotides were inserted into the vector at a 10:1 molar ratio (oligo:vector) using the NEBuilderTM HiFi DNA Assembly Cloning Kit (New England BioLabs, Ipswich, MA, USA), in accordance with the manufacturer’s guidelines. ..

    Article Title: A novel role for the CNTN6 locus in lumenization and radial glial cell fate determination during early human cortical development revealed in cerebral organoids
    Article Snippet: .. Guide RNAs were cloned into a gRNA cloning vector (Addgene, #41824). ..

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. ) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl −1 ) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    In Vitro:

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination.
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. 76) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl−1) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. ) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl −1 ) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Purification:

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination.
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. 76) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl−1) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. ) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl −1 ) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Polymerase Chain Reaction:

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination.
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. 76) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl−1) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Article Title: RNA-guided systems for in vivo gene editing
    Article Snippet: .. Confirmed protospacers were cloned into gRNA cloning vector (world wide website addgene.org/41824/) using overlap extension PCR. .. Different pairs of guide RNA plasmids flanking exon 23 or exons 52 and 53 were co-transfected with Sp hCas9_p2A GFP encoding plasmid into mouse C2C12 myoblast cells using lipofectamine 2000 Reagent (Invitrogen) according to the manufacturer's instructions.

    Article Title: Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination
    Article Snippet: .. The sgRNAs were prepared as in the SpCas9 mRNA preparation, except that: (a) the gRNA cloning vector (Addgene, 41824; ref. ) was used as the template; (b) in vitro transcription was performed using the MEGAshortscript T7 Transcription Kit, in which the mixture contained: 7.5 μl (100 ng μl −1 ) of purified PCR product, 2 μl of T7 10× T7 reaction buffer, 2 μl of T7 ATP solution, 2 μl of T7 CTP solution, 2 μl of T7 GTP solution, 2 μl of T7 UTP solution, 0.5 μl of RNase inhibitor, 2 μl of T7 enzyme mix and 7.5 μl of nuclease-free water; was prepared. ..

    Clone Assay:

    Article Title: RNA-guided systems for in vivo gene editing
    Article Snippet: .. Confirmed protospacers were cloned into gRNA cloning vector (world wide website addgene.org/41824/) using overlap extension PCR. .. Different pairs of guide RNA plasmids flanking exon 23 or exons 52 and 53 were co-transfected with Sp hCas9_p2A GFP encoding plasmid into mouse C2C12 myoblast cells using lipofectamine 2000 Reagent (Invitrogen) according to the manufacturer's instructions.

    Article Title: A novel role for the CNTN6 locus in lumenization and radial glial cell fate determination during early human cortical development revealed in cerebral organoids
    Article Snippet: .. Guide RNAs were cloned into a gRNA cloning vector (Addgene, #41824). ..

    CRISPR:

    Article Title: Light-induced expression of gRNA allows for optogenetic gene editing of T lymphocytes in vivo.
    Article Snippet: .. Other plasmids were obtained from commercial vendors or from the Addgene repository: pRL-CMV-Renilla (Promega), pRS0045 (Addgene #131124), pRS0035 (Addgene #131125), lentiCRISPR v2 (Addgene #52961), pLV-SI-121 (Addgene #131126), pLV-SI-112 (Addgene #131127), pCMV_ABEmax (Addgene #112095), pXR001 (Addgene #109049), pSBtet-GB (Addgene #60504), gRNA-Cloning vector (Addgene #41824), SP-dCas9-VPR (Addgene #63798), CRISPR-SP-Cas9 reporter (Addgene #62733), pCL-Eco (Addgene #12371), pMD2.G (Addgene #12259), and psPAX2 (Addgene #12260). ..



    Similar Products

    church lab grna vector  (Addgene inc)
    96
    Addgene inc church lab grna vector
    Church Lab Grna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/us12595478-4602-8-12
    Average 96 stars, based on 1 article reviews
    church lab grna vector - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    bbsi digested pxr003 vector  (Addgene inc)
    95
    Addgene inc bbsi digested pxr003 vector
    Bbsi Digested Pxr003 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/pXR003%3A+CasRx+gRNA+cloning+backbone+(Plasmid+%23109053)/pm41928225-94-9-12
    Average 95 stars, based on 1 article reviews
    bbsi digested pxr003 vector - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    grna empty vector  (Addgene inc)
    96
    Addgene inc grna empty vector
    Grna Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/10__1093_slash_narmme_slash_ugag003-108-7-10
    Average 96 stars, based on 1 article reviews
    grna empty vector - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    grna cloning vector76  (Addgene inc)
    96
    Addgene inc grna cloning vector76
    Grna Cloning Vector76, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/pm41535291-377-16-27
    Average 96 stars, based on 1 article reviews
    grna cloning vector76 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    grna cloning vector  (Addgene inc)
    96
    Addgene inc grna cloning vector
    Grna Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/pmc12808108-348-16-27
    Average 96 stars, based on 1 article reviews
    grna cloning vector - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    george church laboratory  (Addgene inc)
    96
    Addgene inc george church laboratory
    George Church Laboratory, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/pm41331925-49-17-20
    Average 96 stars, based on 1 article reviews
    george church laboratory - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results